TABLE 1.
Kinetic parameters of TreS substrates
N.D., no turnover detected; N/A, not applicable, since not UV-Vis absorbing.
| Substrate | pKa | Km | kcat | kcat/Km | Δϵ |
|---|---|---|---|---|---|
| mm | s−1 | mm−1s−1 | m−1cm−1 | ||
| DNPGlc | 3.96 | 2.9 ± 0.1 | 4.7 ± 0.1 | 1.6 | 9,800 |
| 34DNPGlc | 5.36 | 2.5 ± 0.1 | (4.3 ± 0.1) × 10−2 | 1.7 × 10−2 | 14,220 |
| 4C2NPGlc | 6.45 | 2.2 ± 0.1 | 8.3 ± 0.2 | 3.8 | 1,300 |
| 4NPGlc | 7.18 | 5.8 ± 0.2 | 0.10 ± 0.01 | 1.7 × 10−2 | 6,464 |
| 2NPGlc | 7.22 | 0.7 ± 0.1 | 8.1 ± 0.3 | 12 | 1,650 |
| 3NPGlc | 8.39 | N.D.a | N.D. | N.D. | 167 |
| aGlcF | 3.17 | 0.15 ± 0.03 | 5.3 ± 0.3 | 35 | N/A |
| Maltose | ∼16 | 8.0 ± 0.9 | 19 ± 1 | 2.4 | N/A |
| Trehalose | ∼16 | 87 ± 6 | 66 ± 2 | 0.75 | N/A |