TABLE 2.
scanc2/AMM mutants growing on a non-fermentable carbon source due to the emergence of compensatory second-site mutations in the scanc2/AMM ORF
TMS, transmembrane segment.
| Revertant strains | ORF position | Condon changes | Amino acid changes | Location |
|---|---|---|---|---|
| scanc2/AMM, G29C | 88 | GGT → TGT | G29C | TMS1 |
| scanc2/AMM, S32N | 98 | AGT → AAT | S32N | TMS 1 |
| scanc2/AMM, A101T | 304 | GCT → ACT | A101T | TMS 2 |
| scanc2/AMM, F104L | 315 | TTC → TTG | F104L | TMS 2 |
| scanc2/AMM, A105T | 316 | GCC → ACC | A105T | TMS 2 |
| scanc2/AMM, A105V | 317 | GCC → GTC | A105V | TMS 2 |
| scanc2/AMM, Y206H | 619 | TAC → CAC | Y206H | TMS 4 |
| scanc2/AMM, G208S | 625 | GGT → AGT | G208S | TMS 4 |
| scanc2/AMM, G297S | 892 | GGT → AGT | G297S | TMS 4 |
| scanc2/AMM, A298S | 895 | GCT → TCT | A298S | TMS 6 |
| scanc2/AMM, I301T | 905 | ATC → ACC | I301T | TMS 6 |
| scanc2/AMM, L307P | 923 | CTG → CCG | L307P | C terminus |