FIGURE 1.

Cytoplasmic localization of hCAR T38D in Huh-7 cells. A, ectopically expressed GFP-hCAR T38A and GFP-hCAR T38D were visualized using a confocal microscope. Hoechst DNA staining was used to visualize the nuclei. Scale bars = 10 μm. B, ectopically expressed FLAG-hCAR T38A or FLAG-hCAR T38D in cytosolic and nuclear extracts was subjected to Western blot analysis with an anti-FLAG antibody. Blots were subsequently stripped and restained with anti-α-tubulin or anti-TATA-binding protein antibody as a loading control. IB, immunoblot.