FIGURE 2.

Specific interaction between hCAR T38D and p-ERK1/2 in Huh-7 cells. As described under “Experimental Procedures,” FLAG-hCAR T38A and FLAG-hCAR T38D were ectopically expressed in Huh-7 cells, from which cell extracts were prepared and incubated with an anti-p-ERK1/2 antibody. The resultant precipitates were analyzed by Western blotting with an anti-FLAG antibody for co-immunoprecipitation (IP) of CAR with p-ERK1/2. Cytosolic extracts were also blotted with an anti-FLAG antibody to confirm expression levels of CAR mutants (2% input). IB, immunoblot.