FIGURE 5.

Dephosphorylation of Thr-38 of hCAR in U0126-treated Huh-7 cells. A, whole cell extracts were prepared from drug-treated Huh-7 cells ectopically expressing FLAG-hCAR or FLAG-hCAR T38D as described under “Experimental Procedures.” Western blotting was performed using anti-phospho-Thr-38, anti-FLAG, anti-p-ERK1/2, and anti-ERK1/2 antibodies. IB, immunoblot. B, ELISA with an anti-phospho-Thr-38 antibody was used for the cytosolic extracts, which were prepared from drug-treated Huh-7 cells ectopically expressing FLAG-hCAR or FLAG-hCAR T38D as described under “Experimental Procedures.” *, significantly different (p < 0.05) from WT CAR treated with U0124 (unpaired t test). ±Error bars represent the mean ± S.E.