Dephosphorylation of CAR in mouse primary hepatocytes after treatment with U0126. Primary hepatocytes were prepared from male C3H mice and treated with phenobarbital (PB; 2 mm), U0124 (20 μm), or U0126 (20 μm) for 1 h. Whole cell and nuclear extracts were then prepared for Western blot analysis with anti-CAR, anti-ERK1/2, anti-p-ERK1/2, and anti-TATA-binding protein antibodies. The TATA-binding protein levels were used as a loading control.