FIGURE 4.

Effect of YnjE on the sulfur transfer from IscS for in vitro formation of MPT. Mixtures containing a 5 μm concentration of the inactive form of MPT synthase, cPMP (in excess), 5 μm MoeB, 2.5 mm Mg-ATP, 0.5 mm l-cysteine, and 2.5 μm IscS dimers were incubated in the absence and presence of 2.5, 5, and 25 μm His₆-YnjEΔ1–21 (white bars) and His₆-YnjEΔ1–21C385A (black bars) as indicated. Mixtures were incubated for 15 min at room temperature, and the amount of MPT formed during the reaction was quantified as Form A using the peak area. Fluorescence was monitored with excitation at 383 nm and emission at 450 nm. Error bars, S.E.