FIGURE 7.

Cellular localization of YnjE. Strains BL21(DE3), pAU2 in BL21(DE3), and MC1061(DE3) were used to determine the cellular localization of YnjE. E. coli cells were grown in LB medium in the presence or absence of 0.2% (w/v) maltose. After centrifugation, the cells were separated into cytoplasmic and periplasmic fractions. A, BL21(DE3) (pAU2) cells were grown in LB medium without isopropyl β-d-thiogalactopyranoside induction and fractionated, and cytoplasmic and periplasmic fractions were loaded in lanes 1 and 2, respectively. BL21(DE3) cells were grown in LB and fractionated, and cytoplasmic and periplasmic fractions were loaded in lanes 3 and 4, respectively. His₆-tagged YnjEΔ1–21 and endogenous YnjE were detected by using polyclonal YnjE antisera. B, MC1061(DE3) cells were grown in LB medium containing 0.2% (w/v) maltose, and YnjE and MBP (which is a periplasmic protein) were detected using polyclonal YnjE (lanes 1 and 2) and MBP (lanes 3 and 4) antisera, respectively. Lanes 1 and 3, cytoplasmic fraction; lanes 2 and 4, periplasmic fraction.