TABLE 1.
E. coli strains and plasmids used in this study
| Plasmid or strain | Genotype or relevant characteristics | Source or reference |
|---|---|---|
| Plasmids | ||
| pJD10 | sufS gene cloned into NdeI and BglII sites of pACYCDuet1, CMR | This study |
| pJD11 | csdA gene cloned into NdeI and BglII sites of pACYCDuet1, CMR | This study |
| pJD12 | sufS gene cloned into NdeI and BamHI sites of pET11b, AmpR | This study |
| pJD13 | csdA gene cloned into NdeI and BamHI sites of pET11b, AmpR | This study |
| pAU1 | ynjE gene cloned into XhoI and XhoI sites of pEB327, AmpR | This study |
| pAU2 | ynjE gene without its periplasmic leader sequence (YnjEΔ1-21) cloned into BamHI and NotI sites of pACYCDuet1, CMR | Ref. 19 |
| pAU3 | ynjEΔ1-21C385A cloned into BamHI and NotI sites of pACYCDuet1, CMR | Ref. 19 |
| pPJ15 | ynjE cloned into EcoRI and XhoI sites of pT7-7 | This study |
| pAF40 | moaD gene cloned into SalI and HindIII sites of pEB327, AmpR | This study |
| pAF41 | moeB gene cloned into SalI and HindIII sites of pEB327, AmpR | This study |
| pET-Ehis | pET22b expressing SufE-His6, AmpR | Ref. 30 |
| pLEC-E | pET22b expressing CsdE-His6, AmpR | Ref. 29 |
| pSL209 | iscS gene cloned into NcoI and BamHI sites of pET15b, AmpR | Ref. 10 |
| pSL213 | sufS gene cloned into NdeI and BamHI sites of pET15b, AmpR | Ref. 10 |
| pSL215 | csdA gene cloned into NdeI and BamHI sites of pET15b, AmpR | Ref. 10 |
| pSL219 | iscS gene cloned into BamHI and BglII sites of pACYC184, CmR | This study |
| pGG110 | moaE gene cloned into NcoI and BglII sites of pQE60, AmpR | Ref. 7 |
| pMW15eB | moeB gene cloned into NcoI and BamHI sites of pET15b, AmpR | Ref. 10 |
| pMW15aD | moaD gene cloned into NcoI and BamHI sites of pET15b, AmpR | Ref. 49 |
| pMW15aDaE | moaDE genes cloned into NcoI and BamHI sites of pET15b, AmpR | Refs. 9 and 49 |
| Strains | ||
| MC1061(DE3) | F−araD139 Δ(ara leu)7696 Δ(lacY74) galU galK hsdR hsdM+ rpsL150(DE3) | Ref. 21 |
| JLD42301 | MC1061 ΔynjE | This study |
| CL100(DE3) | MC1061 ΔiscS | Ref. 21 |
| PJ18(DE3) | MC1061 ΔynjE ΔiscS | This study |
| BL21(DE3) | F−ompT hsdSB(rB−mB−) gal dcm (DE3) | Novagen |