Table 1.
Primer sequences used for the applied PCR protocols.
| PCR | Primer sequence | References |
|---|---|---|
| 16S-rRNA gene PCR | 63f (5′-CAGGCCTAACACATGCAAGTC-3′) 1387r (5′-GGGCGGWGTGTACAAGGC-3′) |
Marchesi et al. (1998) |
| T7_63f (5′-TAATACGACTCACTATAGGGCAGGCCTAACACATGCAAGTC-3′)a SP6_1387r (5′-CATTTAGGTGACACTATAGGGGCGGWGTGTACAAGGC-3′)b | ||
| PLC_PCR | PL3 (5′-AAGTTACCTTTGCTGCATAATCCC-3′) PL7 (5′-ATAGATACTCCATATCATCCTGCT-3′) |
Fach and Popoff (1997) |
| CP_PCR | CP1f (5′-AAAGATGGCATCATCATTCAAC-3′) CP2r (5′-TACCGTCATTATCTTCCCCAAA-3′) |
Wang et al. (1994) |
a
Primer 63f was combined with sequencing primer T7.
b
Primer 1387r was combined with sequencing primer SP6.