Figure 5.

Improved S/N ratio of concentrations of metabolites obtained by least-squares peak fitting. (Blue line) time course generated by PIBS using NMR operation software Vnmrj (Palo Alto, CA). (Red/black lines) MPSF using selected windows (Fig. 3C). The black line denotes the mean, and red lines denote 2-σ error bars (see Experimental Procedures for the error bar determination method). Fitting peaks in multiple windows by MPSF gives an order of magnitude improvement in concentration estimation.