Figure 3. Energy dissipation curves of E. coli aspartokinase III.

(A) Energy dissipation curve on condition that the velocity of Ser345 is increased by four fold (blue line) and the two-state Boltzmann equation fitting result of the energy dissipation curve (red line). NR' is the first derivation at the center point. (B) Energy dissipation curves on condition that the velocity of Ser345 is increased by different folds. (C) Energy dissipation curves on condition that the velocity of different residues is increased by four fold. R stands for the case when the velocities of all of the three key residues are increased by four fold at the same time.