Fig. 4.

Change in BrdU-retaining cell number after ischemia/reperfusion injury. Twelve hours after birth, mice were administered with 50 mg/kg body weight of BrdU, ip, for 6 days. Eight weeks after the chase period, mouse kidneys were subjected to 30 min of bilateral ischemia and harvested 5 and 16 days after ischemia. (A-C) Kidney sections were immunostained using anti-BrdU antibody, and images were taken at low (A) and high (B, C) magnifications. (B and C) are high magnification images of the left and right dot boxes of (A), respectively. Scale bars=250 µm. (D) BrdU-positive cells were counted. Results are expressed as the mean±SE (n=4). ^*P<0.05 vs. the deep cortex and inner medulla of day 0 in Fig. 3, ^†P<0.05 vs. the inner medulla at respective time point.