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. Author manuscript; available in PMC: 2012 Oct 12.
Published in final edited form as: Structure. 2011 Sep 1;19(10):1485–1495. doi: 10.1016/j.str.2011.07.016

Figure 6. Phe105 loop Bro1 mutants bind HIV-1 NCp6 domain.

Figure 6

GST, GST-NCp6 (A) or GST NC-p6 RKI mutant (B) fusion proteins were expressed in E. coli, captured on Glutathione conjugated beads and then subsequently incubated with lysates from 293T cells expressing WT HA-Bro1 domain or the indicated Phe105 loop Bro1 mutants. Captured proteins and cell lysates were analyzed by SDS-PAGE and western blot using anti-HA antibody. GST fusion proteins were visualized by Coomassie blue staining.