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. Author manuscript; available in PMC: 2012 Oct 12.
Published in final edited form as: Structure. 2011 Sep 1;19(10):1485–1495. doi: 10.1016/j.str.2011.07.016

Figure 7. The Phe105 loop mutants retain binding to CHMP4B.

Figure 7

The Phe105 loop mutant versions of Bro1 domain (A) or full-length Alix (B) were tested for their ability to bind CHMP4B in immunoprecipitation assays. 293T cells were co-transfected with Flag-tagged CHMP4B alone (lane 1), or in combination with WT HA-Bro1, HA-Alix (lane 2), or the HA tagged indicated mutant (lanes 3–8). Cells were lysed in RIPA buffer and clear lysates were incubated with anti-HA antibody-conjugated beads. Both input and immunoprecipitated complexes were analyzed by SDS-PAGE and western blot using the indicated antibodies.