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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Jul 23;1808(12):2948–2953. doi: 10.1016/j.bbamem.2011.07.008

FIGURE 2.

FIGURE 2

(a) Analysis of key residues in the DAGK TMD by Multi-Tox. Error bars represent the standard deviation of three replicates, each measured in quadruplicate. Inset: classification of DAGK mutants for disruption of trimerization as previously reported [15]; (b) Western blots of protein expression for DAGK TMD; (c) Cartoon representation of the MBP-DAGK-ToxR Multi-Tox chimeric protein, summarizing the DAGK residues investigated. DAGK residues are numbered according to the wild-type unmodified DAGK protein. Arrows indicate direction of numbering for each helix turn.