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Dominant-negative (DN) ToxR and Multi-Tox assays of LMP-1 constructs for analysis of heterotypic interactions. (a) Interaction of ToxR-TM5 with signaling-deficient ToxR*-TM5; (b) Interaction of ToxR-TMD456 with signaling deficient ToxR*-TMD456; Western blots for (c) the DN-ToxR assay and (d) the DN-Multi-Tox assay. Control plasmid does not contain either the ToxR or the MBP reading frame. Error bars represent the standard deviation of three replicates, each measured in quadruplicate.
