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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Gen Comp Endocrinol. 2011 Sep 1;174(2):202–210. doi: 10.1016/j.ygcen.2011.08.019

Fig. 1.

Fig. 1

Cloning and expression of the cDNAs for Panx1 isoforms from rat pituitary primary cells. (A) Nest RT-PCR was applied for amplification of the rat Panx1 cDNAs (A). 1st, the first round PCR products using primer pair Panx1F/Panx1R; 2nd, the second round nest PCR products using 100 times dilution of the first round PCR products as template and primer pair Panx1F1/Panx1R1. (B) Schematic representation of primer pairs used for detection of different Panx1 splicing variants. (C) The presence of Panx1c and Panx1d specific mRNA transcripts in normal and GH3 immortalized pituitary cells. The sizes of PCR products were as follows: 790 bp for Panx1c vs. 1.0 kb for Panx1a in detection of Panx1c isoform; 428 bp for Panx1d vs. 711 bp for Panx1a/c in detection of Panx1d isoform. 1: DNA marker; 2: GH3 cells cDNA; 3: pituitary cells cDNA; 4: plasmid control. Right panels show DNA markers.