Cellular localization of Panx1 isoforms. HEK293 and GT1 cells were transiently transfected with FLAG-tagged Panx1a, Panx1c and Panx1d constructs. Cells were fixed, permeabilized, and fluorescence was analyzed 36 h after transfection using confocal laser scanning microscopy. Note that FLAG-tagged Panx1a, but not FLAG-tagged Panx1c and Panx1d isoforms, was expressed on the cell surface in both cell lines. Bar: 5 µm.