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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Gen Comp Endocrinol. 2011 Sep 1;174(2):202–210. doi: 10.1016/j.ygcen.2011.08.019

Fig. 6.

Fig. 6

The biotin labeling analysis of the cell surface localization of Panx1 isoforms. HEK293 cells were transfected with N-terminal FLAG-tagged Panx1 constructs and 48 h later labeled with the membrane impermeable Sulfo-NHS-SS-Biotin. Total cell lysates (T) and biotin-labeled plasma membrane protein fractions (BL) were probed with anti-FLAG HRP-labeled antibody (top panel) and the same membranes were reprobed with anti-alpha tubulin antibody (controls, bottom panel). Note that only Panx1a was successfully biotinylated.