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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Gen Comp Endocrinol. 2011 Sep 1;174(2):202–210. doi: 10.1016/j.ygcen.2011.08.019

Fig. 8.

Fig. 8

Association of Panx1 proteins with P2XRs. HEK293 cells were co-transfected with FLAG- tagged Panx1a, Panx1c and Panx1d and V5-tagged P2X7R, P2X2R, P2X3R and P2X4R plasmids. Cell lysates were immunoprecipitated (IP) with anti-V5 antibody (A), anti-P2X2, P2X3, P2X4 or P2X7 antibodies (B) and immunoblotted with anti-FLAG HRP-labeled antibody (WB). +, IP with anti-V5 antibody (A) or anti-P2X2, P2X3, P2X4 and P2X7 antibodies (B); −, IP with normal mouse IgG (A) or normal rabbit serum (B) as negative controls.