Panx1 splice forms and ATP release. AtT-20 pituitary cells were transfected with control plasmid, Panx1a, Panx1c, Panx1d or co-transfected Panx1a with Panx1c or Panx1d (1:1) for 48 h. Panx1 channels were then activated by hypotonic stress for 10 min and the extracellular ATP content was determined as described in Material and Methods. Data shown are normalized mean ± SEM values, showing controls as 100%.