Table 2.
Analysis of transplant site and seeding density in porcine islet transplants on scaffolds.
Transplant Site | Seeding Density | Lectin/islet area | Ki67(+) nuclei/islet area | ||||
---|---|---|---|---|---|---|---|
Slides (n) | Mean | SEM | Slides (n) | Mean | SEM | ||
OM | 1k | 16 | 2.21 | 0.24 | 9 | 2.57 | 0.19 |
OM | 4k | 10 | 2.51 | 0.19 | 6 | 3.22 | 0.59 |
OM | 10k | 20 | 1.35 | 0.25 | 12 | 2.29 | 0.23 |
GSM | 10k | 10 | 0.95 | 0.17 | 7 | 2.12 | 0.15 |
Normalized lectin-positive/insulin-positive areas and Ki67-positive nuclei/insulin-positive areas were calculated. Lectin density was higher (p<0.05) for islets seeded at 1k and 4k in the OMF than at 10k in the OMF or GSM. No significant difference in the number of Ki67-positive nuclei/insulin-positive area between conditions was observed.