Figure 3.
Effects of openers of KCa2.1-2.3 and KCa3.1 in eGFP-positive astrocytes patch clamped in the whole-cell configuration. (A) Typical (time-matched) control time-course indicating currents at the 0 mV point of voltage ramps plotted as a function of time. (B) Typical time-course showing an increase in outward current ∼7 min after application of NS309 [arrowheads indicate time points of the current-voltage relationships shown in (C)]. (C) Current-voltage relationship (1 s voltage ramp) showing selected full traces from the cell described in (B) under control conditions and after exposure to NS309. (D) Typical time course showing lack of induction of current by CyPPA [arrowheads indicate the time points at which the current-voltage relationships shown in (E) were obtained under control conditions and after exposure to CyPPA]. (F) Mean (±SEM) currents at 0 mV normalized to initial control current level. In the presence of 200 nM [Ca2+]i NS309 (500 nM; n = 8) evoked a significant current increase whereas CyPPA (30 µM) was without effect (n = 9) (one-way anova with post hoc Bonferroni's multiple comparison test ***P < 0.0001).