Figure 6.

(A) Representative bands of total and phosphorylated Akt and p38 MAPK detection by Western blot in cultured neonatal rat ventricular cardiomyocytes incubated with noradrenaline (NA) alone, or upon co-incubation with moxonidine at 10⁻⁷ and 10⁻⁵ mol·L⁻¹, without and with I₁-receptor antagonist, AGN 192403 (AGN) at 10⁻⁵ mol·L⁻¹. (B) Bargraph represents ratios of phospho-Akt to total Akt and phospho-p38 to total p38, compared with GAPDH (loading control) and presented as per cent corresponding untreated cells. n = 10–12 wells per treatment from three independent cultures. †P < 0.01 versus Dulbecco's modified Eagle's medium (DMEM) + fetal bovine serum (FBS), 0.1%; ‡P < 0.05 versus NA; &P < 0.05 versus corresponding NA + moxonidine.