Figure 6.

Effects of kaempferol on the LPS-induced expression of MMP-3 and MMP-9, and involvement of MMP-3 in LPS-induced iNOS expression in BV2 cells. Cells were pretreated with 100 µM kaempferol for 1 h, followed by 1.0 µg·mL⁻¹ LPS for 24 h. (A) Zymographic analysis of MMP-3 and MMP-9 activity. The caseinolytic activity of MMP-3 (top), and the gelatinolytic activity of MMP-9 (bottom). (B) Western blot analysis of expression of MMP-3, and MMP-9. Actin was used as an internal loading control. (C) BV2 cells were pretreated with NNGH, a specific inhibitor of MMP-3 for 1 h, followed by LPS for 24 h. Expression of the iNOS was detected by immunoblot assay. Con, control; Kae, kaempferol.