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. Author manuscript; available in PMC: 2012 Aug 31.
Published in final edited form as: Cell Immunol. 2011 Aug 31;271(2):488–495. doi: 10.1016/j.cellimm.2011.08.019

Figure 1.

Figure 1

Mast cell serotonin release induced by pollen extract.

a. 3H-serotonin release induced by pollens extracted in NH4HCO3 buffer. Total protein content = 100 μg/ml. Iono = ionomycin (1 μM), cedar = mountain cedar, rag = ragweed, pig = pigweed, and timothy = timothy grass.

b. 3H-serotonin release was determined in RBL-2H3 cells at 1, 5, 10, 20, and 30 minutes after stimulation with cedar pollen extract (open boxes; □) or 1 μM ionomycin (filled triangles; ▲). Data represent the means of 3 independent experiments, * indicates significant differences between ionomycin and cedar extract ( p< .05).

c. 3H-serotonin release was determined in RBL-2H3 cells after incubation with 25, 50, 100, or 200 μg/ml cedar pollen extract for 30 minutes. The results are expressed as mean ± SD (n = 3 separate experiments, each experiment performed in duplicate).

d. 3H-serotonin release in RBL-2H3 cells after exposure to pollen extract plus DNP-BSA 1 ng/ml (open triangles; △) or pollen extract alone (open boxes; □). DNP-BSA 50 ng/ml (filled circle; ●).