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. Author manuscript; available in PMC: 2012 Aug 31.
Published in final edited form as: Cell Immunol. 2011 Aug 31;271(2):488–495. doi: 10.1016/j.cellimm.2011.08.019

Figure 4.

Figure 4

Serotonin release and ROS generation in mast cells exposed to cedar pollen extracted in various buffers and pHs. RBL-2H3 cells were incubated with cedar pollen extracts adjusted to 100 μg/ml protein, pH 7.3 for 30 minutes and assessed for 3H-serotonin release with results expressed as percent serotonin release (a. and c.), and DCF fluorescence expressed as mean fluorescence ratios to buffer controls (b. and d.).

a. 3H-serotonin release induced by cedar pollen extracted in PBS, NaHCO3,NH4CL, or NH4HCO3.

* indicates significant difference from PBS (p<.05), ** (p<.01)

b. DCF fluorescence induced by cedar pollen extracted in PBS, NaHCO3, NH4CL, or NH4HCO3.

* indicates significant difference from PBS (p<.05), ** (p<.01)

c. 3H-serotonin release induced by cedar pollen extracted in PBS (open bars) or NaHCO3 (filled bars) at pH of 6.4, 7.2, or 8.0.

d. DCF fluorescence induced by cedar pollen extracted in PBS (open bars) or NaHCO3 (filled bars) at pH of 6.4, 7.2, or 8.0.