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. Author manuscript; available in PMC: 2012 Aug 31.
Published in final edited form as: Cell Immunol. 2011 Aug 31;271(2):488–495. doi: 10.1016/j.cellimm.2011.08.019

Figure 5.

Figure 5

Effect of antioxidants and extracellular calcium on serotonin release and ROS generation.

a. and b. RBL-2H3 cells were exposed to either buffer control (filled bars), 1% DMSO (shaded bars), 10 mM NAC (open bars), or, 10 mM TMTU (cross-hatched bars) for 1 hour, washed with medium and then exposed to cedar pollen extract (100 μg/ml protein) for 30 minutes and DCF fluorescence (a.) and 3H-serotonin release (b.) assessed. The results are expressed as mean ± SD (n = 3 experiments). * indicates significant difference (p< .05) from buffer control, ** (p< .01).

c. 3H-serotonin release was assessed in RBL-2H3 cells incubated with ionomycin (1 μM) or 100 μg/ml pollen extract for 30 minutes in the presence or absence of 1.26 mM Ca2+ and/or 5 mM EDTA (indicated below the figure). The results are expressed as mean ± SD (n = 3 experiments).

* indicates significant difference (p< .05) from medium containing Ca2+ and no EDTA, ** (p< .01).