Figure 5.

Resistance to chemotherapy in non-combinatorial, AMD3100-treated MSC/BCC cocultures. BCCs cultured in the presence or absence of MSCs for 12 days were treated for 48 h with AMD3100, or untreated. Following 48 h, treated and untreated cells were exposed to carboplatin for 72 h to induce cell death. The treated cells did not receive chemotherapy in combination with a second dose of AMD3100 (as illustrated by +/-). At the end of this regiment cell proliferation (A and B) and viability (C and D) were assessed. The results are presented as the mean of ten replicates in five different experiments, each with MSCs from a different donor (± SD).