Figure 5.

Model describing the effect of ZD6474 inhibitor on RET/β-catenin interaction. A. When Ret/PTC expressing cells are treated with ZD6474 inhibitor, Ret/PTC is inactivated, therefore more β-catenin can be recruited by E-cadherin to the membrane changing morphology and migration ability of cells. B. Real Time PCR analysis of normalized expression of c-Myc and cyclin D1, β-catenin target genes in TPC1 cells treated with 5 mM ZD6474 (red) and not-treated cells (blue). Jun B, that is not a β-catenin target gene, was used as control. Results are normalized to endogenous GAPDH expression used as control and are presented as the mean ± S.D. (n = 3) of a single experiment, representative of three independent repeats.