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mRNA expression levels of A2b receptor after transient transfection of the Mix siRNA constructions into HepG2 cell lines by RT-PCR. Compared to normal controls, transfection with pSilencer3.1-mix significantly reduced the mRNA of the A2b receptor. The pSilencer3.1-Y did not affect the mRNA level of A2b receptor. β-actin was amplified to verify equal loading.
