Table 1.
Normalized data for the expression of hPer2 and hBmal1 in human peripheral blood mononuclear cells during baseline and sleep-deprived conditions*
| Conditions |
Variable |
Mesor ± SE |
Amplitude ± SE |
Acrophase ± SE (hours) |
ANOVA |
||
|---|---|---|---|---|---|---|---|
| F-value | P | df | |||||
| Baseline |
hPer2/36B4† |
1.20 ± 0.09 |
0.41 ± 0.12 |
13:55 ± 1:15 |
19.7 |
0.001 |
30 |
|
hBmal1/36B4 |
1.29 ± 0.10 |
0.42 ± 0.15 |
16:08 ± 1:18 |
17.6 |
0.001 |
30 |
|
| Sleep-deprived |
hPer2/36B4† |
1.24 ± 0.15§ |
0.15 ± 0.15§ |
11:56 ± 4:35§ |
9.2 |
0.001 |
30 |
| hBmal1/36B4‡ | 1.34 ± 0.11 | 0.36 ± 0.17 | 17:13 ± 1:35 | 13.2 | 0.001 | 30 | |
*Abbreviations: SE – standard error; ANOVA – analysis of variance; df – degrees of freedom.
†Expression of hPer2 as a relative ratio of the constitutively expressed 36B4 gene.
‡Expression of hBmal1 as a relative ratio of the constitutively expressed 36B4 gene.
§Statistically not significant oscillation.