Table 2.
Normalized data for the expression of hPer2 and hBmal1 in human peripheral blood mononuclear cells for each individual during baseline and sleep-deprived conditions. Statistically significant amplitudes (ie, 95% confidence interval does not include zero) are in bold
Participant | Baseline conditions |
Sleep deprived conditions |
||||||
---|---|---|---|---|---|---|---|---|
hPer2/36B4* |
hBmal1/36B4† |
hPer2/36B4* |
hBmal1/36B4† |
|||||
amplitude | acrophase (hours) | amplitude | acrophase (hours) | amplitude | acrophase (hours) | amplitude | acrophase (hours) | |
S1 |
0.7368 |
12:37 |
0.8628 |
14:52 |
0.5830 |
16:34 |
0.8172 |
17:27 |
S2 |
0.6474 |
15:05 |
0.4193 |
16:10 |
0.5342 |
10:56 |
0.5646 |
17:03 |
S3 |
0.3419 |
11:27 |
0.2051 |
18:16 |
0.2850 |
13:37 |
0.3413 |
14:54 |
S4 |
0.2178 |
16:03 |
0.5830 |
18:53 |
0.3547 |
21:36 |
0.5386 |
21:12 |
S5 |
0.1939 |
13:14 |
0.3304 |
14:42 |
0.2323 |
19:27 |
0.1878 |
17:16 |
S6 | 0.5165 | 15:01 | 0.3727 | 15:35 | 0.7344 | 6:14 | 0.2549 | 10:45 |
*Expression of hPer2 as a relative ratio of the constitutively expressed 36B4 gene.
†Expression of hBmal1 as a relative ratio of the constitutively expressed 36B4 gene.