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. Author manuscript; available in PMC: 2012 Oct 13.
Published in final edited form as: Eur J Neurosci. 2011 Oct 13;34(8):1200–1211. doi: 10.1111/j.1460-9568.2011.07857.x

Fig. 3.

Fig. 3

AnxA2 – an NR1 3′-UTR-binding trans-acting protein. A 47 kDa protein band in gel obtained using polysomes from ethanol-exposed CC was processed for in-gel trypsin digestion. Tryptic fragments were analyzed by microcapillary reverse-phase HPLC nano-electrospray tandem mass spectrometry and MS spectra were correlated with known proteins using two different programs. (A) Amino acid sequence of peptides that matched 100% to AnxA2 (gi|18645167). (B) Amino acid sequence of murine AnxA2 (339 residues). Shaded boxes with bold letters indicate the amino acid sequence that matched with eight peptides. Two peptides that spanned the same region of AnxA2 are highlighted by a double underline. Comparison of amino acid sequence of eight matched peptides with AnxA2 demonstrated a 27% sequence coverage.