Figure 4.

Inhibition of biotin-labeled cell surface proteins. Eluates of WS-1 cells untreated (−) or transduced with pHSVsiLA1 or the scrambled control vector pHSVsiNC, examined for biotinylated GABA α1 protein by immunoblotting. Approximately 50% of the total levels of α1 protein expressed in the cells is biotinylated (i.e., expressed on the cell surface). Similar levels of biotinylated α1 protein were seen in cells treated with pHSVsiNC, but treatment with pHSVLA1 caused a significant decrease in the levels of biotinylated α1 protein [F(3,8) = 316.78, P < 0.001]. Subsequent Tukey post hoc test confirmed that inhibition of α1 expression by pHSVsiLA1 also caused a significant reduction in the levels of cell surface protein expression (P < 0.01).