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. 2011 Oct 18;5:123. doi: 10.3389/fnins.2011.00123

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Copyright © 2011 Yang, Liu, Yi, Warnock, Wang, June, Puche, Elnabawi, Sieghart, Aurelian and June.

This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with.

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pHSVsiLA1 delivery into the VP inhibits alcohol intake in HAD rats. (A–C) HAD rats were microinfused with pHSVsiLA1 (n = 6) or PBS (control; n = 6) into the VP and examined for intake of alcohol (10% v/v) (A), water (B), or sucrose (C) in a home-cage bottle paradigm for 90 min. Alcohol results are expressed in grams per kilogram (g/kg). Prior to surgery, BACs in the PBS control and pHSVsiLA1 groups were 112 mg%/dL ± 13 and 118 mg%/dL ± 25, respectively. Two-way ANOVA revealed significant main effects of Day [F(28,140) = 5.58, P < 0.001], Group [F(1,5) = 86.184, P < 0.001], and a significant interaction for Day × Group [F(28,140) = 5.35, P < 0.001]. Relative to the control group, significant reductions in alcohol drinking were observed in the pHSVsiLA1-treated group from days 3–25 (Tukey post hoc test). Alcohol intake was similar during days 26–30 for the pHSVsiLA1 and PBS control groups. (B) Water intake (mL/kg)/22.5 h was also similar across pre-surgical days and post-surgical days 1–10 between the pHSVsiLA1 and PBS control groups. (C) Sucrose intake in the two-bottle choice home-cage paradigm over the 90-min period. ANOVA revealed a significant session effect [F(7,74) = 3.616, P < 0.0001]. Specifically, both the control and pHSVsiLA1 pre-surgery groups demonstrated significantly higher drinking than the post-surgery groups on day 3 (P < 0.01). However, no differences were observed between the two groups at pre-surgery or at any post-surgery time point (P > 0.01). (D) HAD rats were given the scrambled amplicon pHSVsiNC (n = 6) or PBS (control; n = 6) into the VP and examined for alcohol consumption. Results are expressed in grams per kilogram (g/kg) during the daily 90 min alcohol sessions. Prior to surgery, BACs in the PBS and pHSVsiNC groups were 133 mg%/dL ± 26 and 122 mg%/dL ± 21, respectively. ANOVA revealed a significant session effect [F(7, 80) = 11.96, P < 0.0001], but no group effects. Specifically, drinking in the both the control and pHSVsiNC pre-surgery groups was significantly greater than in the post-surgery groups on days 3 and 4 (P < 0.01). However, no differences were observed between the two groups at pre-surgery or at any post-surgery time point (P > 0.01). Significant effects were identified using the Newman–Keuls post hoc test.