Fig. 3.
Inhibition of AMPK and SIRT negates Leptin’s effect on Aβ(1–40) in RA-SY5Y. Cells were treated overnight with or without Leptin (500 ng/mL) in the presence or absence of nicotinamide (5 mM) or compound C (20 μM), and culture media collected for determination of Aβ(1–40) levels by ELISA. Results were normalized to total protein and presented as a percentage relative to vehicle control, n=3.
*p<0.05 vs. no Leptin