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Steady-state kinetics of L- -lysine activation by B. cereus LysRS wild type and variants.
| KM (μM) | kcat (min−1) | kcat/KM(min−1/μM) | |
|---|---|---|---|
| Wild Type | 160 ± 70 | 2500 ± 30 | 16 |
| A233S | 210 ± 60 | 5100 ± 490 | 24 |
| G469A | 3900 ± 1200 | 490 ± 30a | 0.13 |
| A233S/G469A | NDb |
Errors correspond to the standard deviation of three independent experiments.
Based on estimated active yield, see text for details.
Kinetic parameters for the LysRS variant A233S/G469A could not be determined.
