FIGURE 4.

Only mature neurons promote glutamate-induced CDKL5 efflux into the cytoplasm. A, Western blot analysis of CDKL5 expression in hippocampal embryonic neurons cultured for the indicated times. β-Tubulin III (TUJ1) was used as internal standard. B, hippocampal neurons were cultured for 3 (DIV 3), 7 (DIV 7), and 14 (DIV 14) days before a 10-min treatment with 10 μm glutamate (Glut); control neurons are indicated with KRH. Fixed neurons were then stained for CDKL5 (left panels) and DAPI (right panels). In these representative images the scale bar is 10 μm.