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. 2011 Aug 23;286(42):36852–36863. doi: 10.1074/jbc.M111.219931

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Survival of the invA⁻ mutant, invA^com revertant, and wild-type (WT) of L. interrogans strain Lai in hamsters. A, leptospires of the invA⁻ mutant, invA^com revertant, and wild type in hamster monocytes for the indicated times. Blue: nucleus; green: cytoplasm; red: leptospires. B, statistical summary of fluorescence intensity (as in A) reflecting internalization levels of the invA⁻ mutant, invA^com revertant, and wild type in hamster monocytes for the indicated times. 100 cells of each strain were analyzed to quantify the fluorescence signal intensity. *: p < 0.05 versus the wild type and invA^com revertant. C, leptospires in urine of hamsters infected with the invA⁻ mutant, invA^com revertant, and wild-type (Fontana silver staining). D, counts of leptospires of the invA⁻ mutant, invA^com revertant, and wild type in urine of hamsters at the indicated times. *: p < 0.05 versus the wild type and invA^com revertant.