FIGURE 11.

N-terminal mutations of CDK-phosphorylated Sld2 that inhibit binding to ssARS1-1. A and B, 3 pmol of CDK-phosphorylated GST-Sld2, full-length or fragments, was used to pull down varying amounts of ssARS1-1. The results were analyzed by SDS-PAGE followed by phosphorimaging. C, 3 pmol of CDK-phosphorylated GST-Sld2-1-106, wild-type or mutants, was used to pull down varying amounts of ssARS1-1. D, 2 pmol of CDK-phosphorylated GST-Sld2-1-106, wild-type or mutants, was used to pull down varying amounts of radiolabeled Dpb11.