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. 2011 Aug 24;286(42):36414–36426. doi: 10.1074/jbc.M111.282822

FIGURE 12.

FIGURE 12.

C-terminal mutation of CDK-phosphorylated Sld2 that inhibits binding to ssARS1-1. A, 3 pmol of CDK-phosphorylated GST-Sld2 fragments were used to pull down varying amounts of ssARS1-1. The results were analyzed by SDS-PAGE followed by phosphorimaging. B, 3 pmol of CDK-phosphorylated GST-Sld2–79-453, wild-type or mutants, was used to pull down varying amounts of ssARS1-1. Mutants 1-7 are: 1, K416E; 2, K420E,R421E,K422E; 3, R424E,K425E,K426E; 4, R435E,R436E; 5, K441E,K442E; 6, R444E; 7, R449E,R452E,R453E. C, 2 pmol of CDK-phosphorylated GST-Sld2–79-453, wild-type or mutants, was used to pull down varying amounts of radiolabeled Dpb11.