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ssARS1-1 induces the dissociation of CDK-phosphorylated Sld2 from Mcm2–7. A, 2 pmol of CDK-phosphorylated GST-Sld2, GST-Sld2, or GST-Sld2T84D was incubated with 2 pmol of radiolabeled Mcm2–7 in the presence of varying concentrations of ssARS1-1 or ssARS1-2. The results were analyzed by SDS-PAGE. B, results from experiments like A were averaged and plotted.
