FIGURE 5.

Yra1 RRM mutations in S. cerevisiae cause growth defects. A, the RRM point mutations (R107A, Y112A, F126A, R107A/F126A, and Y112A/F126A) and the N-terminal truncated protein (77–226) were expressed in a YRA1-deleted yeast strain. Serial 10-fold dilutions of cells were spotted on rich medium (YPD plates) and incubated at 30 °C (left), 37 °C (middle), or 16 °C (right). B, in situ localization of Yra1 proteins in yeast. V5 epitope-tagged Yra1 constructs were detected by immunofluorescence using a FITC-labeled anti-V5 antibody (green); DAPI (blue) was used to stain the nuclei.