FIGURE 1.

Characterization of Nelf-b deletion in MEFs. A, genotyping verification of Cre-mediated genetic ablation of Nelf-b. MEFs were infected with control or Cre-expressing retrovirus. To rescue the observed effect of Nelf-b deletion, MEFs were also coinfected with a retrovirus that expressed FLAG-tagged Nelf-b. Nelf-b deletion was verified by PCR with primer pairs specific to the floxed (f) and deleted allele (Δ). B, Western blot showing protein levels of Nelf-b and α-tubulin in control and Nelf-b KO cells, with and without ectopic Nelf-b. C, immunostaining of Nelf-b in control and Nelf-b KO MEFs. ToPro was used to visualize the nuclei.