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. 2011 Aug 26;286(42):36215–36227. doi: 10.1074/jbc.M111.246116

FIGURE 3.

FIGURE 3.

MITF binding and activity at E-box sites. A, ChIPs with anti-MITF or control rabbit IgG in proliferating T98G cells treated with DMSO, LY294002, or PI-103 for 30 min. Data are the mean ± S.E. of 3 and 2 samples for LY294002 and PI-103 treatments, respectively. Binding of MITF was significantly higher than IgG controls upstream of ATROGIN-1 and CCNG2 after LY294002 or PI-103 treatment (p < 0.02). B, T98G cells were transfected with either MITF siRNA or nonspecific control siRNA for 48 h followed by 4 h of treatment with either DMSO or LY294002. Knockdown of MITF protein was 75–80% (see inset). Data represent the % induction after LY294002 treatment in cells transfected with MITF siRNA compared with the nonspecific control siRNA and are the mean ± S.E. of 4 independent transfections. The induction of ATROGIN-1 and CCNG2 was significantly lowered after MITF knockdown (*, p < 0.05).