FIGURE 1.

Interaction of Necl-2 with integrin α₆β₄. HEK293 cells were co-transfected with various combinations of the indicated plasmids and cultured in suspension. FLAG-tagged Necl-2 was immunoprecipitated using the anti-FLAG mAb (A, panel a, B, and C) or integrin β₄ was immunoprecipitated using the anti-integrin β₄-mAb (A, panel b), and samples were subjected to Western blotting using the indicated Abs. A, panels a and b, FLAG-Necl-2 and integrin α₆β₄; B, FLAG-Necl-2 and integrin α₆β₁; C, FLAG-Necl-2 and integrin α₅β₁. IB, immunoblot.