Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Aug 31;286(42):36667–36676. doi: 10.1074/jbc.M110.200535

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 2. — Interaction of Necl-2 with integrin α₆β₄ through its extracellular region. A, schematic diagram of Necl-2 fragments. Necl-2 fragments were tagged with a FLAG epitope at the N terminus. FERM, 4.1/ezrin/radixin/moesin. +, positive for the interaction with integrin β₄; −, negative for the interaction with integrin β₄. B, HEK293 cells were co-transfected with various combinations of the indicated plasmids and cultured in suspension. FLAG-tagged full-length Necl-2 or deletion mutants of Necl-2 were immunoprecipitated using the anti-FLAG-mAb, and samples were subjected to Western blotting using the indicated Abs. Asterisks, full-length or mutants of Necl-2. IB, immunoblot.