FIGURE 3.
Interaction of integrin α6β4 with Necl family proteins. A, HEK293 cells were co-transfected with various combinations of the indicated plasmids and cultured in suspension. Each FLAG-tagged Necl family protein was immunoprecipitated using the anti-FLAG mAb, and samples were subjected to Western blotting using the indicated Abs. B, the band intensity of immunoprecipitated integrin β4 was first normalized against that of immunoprecipitated Necl family members, and then the relative values were represented in the graph by setting a value of the Necl-2-immunoprecipitated integrin β4 as 1. Error bars indicated the means ± S.E. of three independent experiments. IB, immunoblot.